GeneTex
United States (US)

West Nile virus NS1 protein antibody

GTX132053_42151_20180525_WB_WNV_18121410_624.jpg
GTX132053_42151_20160616_WB_B_18121410_766.jpg
GTX132053_42151_20160317_WB_WNV_DTV_JEV_18121410_753.jpg

Cat No. GTX132053

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity West Nile virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Positive Control

WNV-infected 293T , WNV-infected vero cell , JEV-infected vero cell , WNV NS1-transfected 293T cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.57mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of West Nile virus NS1 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

WestNile virus NS1 protein antibody, WestNile virus (WNV) NS1 antibody, WNV NS1 antibody

Research Area

DATA IMAGES
GTX132053_42151_20180525_WB_WNV_18121410_624.jpg

GTX132053 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus NS1 protein antibody (GTX132053) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132053_42151_20160616_WB_B_18121410_766.jpg

GTX132053 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile virus NS1 protein antibody (GTX132053) diluted at 1:10000.

GTX132053_42151_20160317_WB_WNV_DTV_JEV_18121410_753.jpg

GTX132053 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus NS1 protein antibody (GTX132053).

REVIEW

West Nile virus NS1 protein antibody Cat No. GTX132053

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts. Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, overnightHr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169