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Wilms Tumor 1 antibody

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563
Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563
Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563
Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563
Anti-Wilms Tumor 1 antibody used in Western Blot (WB). GTX100563

Cat. No. GTX100563

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Wilms Tumor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Human, Chicken, Pig, Japanese Medaka, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wilms Tumor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241456

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WT1 transcription factor , AWT1 , GUD , NPHS4 , WAGR , WIT-2 , WT33

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563

GTX100563 IHC-P Image

Wilms Tumor 1 antibody detects WT1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563

GTX100563 IHC-P Image

Wilms Tumor 1 antibody detects WT1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563

GTX100563 IHC-P Image

Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100563

GTX100563 IHC-P Image

Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wilms Tumor 1 antibody used in Western Blot (WB). GTX100563

GTX100563 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with wt1a + wt1b antibody (GTX100563) diluted at 1:1000.

REFERENCE

Application Reference

Jing Qiao et al. Carbohydrate Polymers 2018; (Epub) Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169