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XLF antibody

Anti-XLF antibody used in Western Blot (WB). GTX64572
Anti-XLF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64572
Anti-XLF antibody used in IHC (Paraffin sections) (IHC-P). GTX64572
Anti-XLF antibody used in IHC (Paraffin sections) (IHC-P). GTX64572
Anti-XLF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64572

Cat. No. GTX64572

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-homologous end joining factor 1 , XLF

Cellular Localization

Nucleus

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-XLF antibody used in Western Blot (WB). GTX64572

GTX64572 WB Image

WB analysis of various sample lysates using GTX64572 XLF antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-XLF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64572

GTX64572 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64572 XLF antibody.
Blue : DAPI
Dilution : 1:100

Anti-XLF antibody used in IHC (Paraffin sections) (IHC-P). GTX64572

GTX64572 IHC-P Image

IHC-P analysis of human uterine cancer tissue using GTX64572 XLF antibody.
Dilution : 1:100

Anti-XLF antibody used in IHC (Paraffin sections) (IHC-P). GTX64572

GTX64572 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX64572 XLF antibody.
Dilution : 1:100

Anti-XLF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64572

GTX64572 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64572 XLF antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399