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XRCC3 antibody [XRCC3 10F1/6]

Cat No. GTX40254

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 XRCC3 10F1/6

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μg ($339)
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APPLICATION

Application Note

For WB: Use at an assay dependent dilution. Detects a band of approximately 37.8 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

38 kDa. ( Note )

Positive Control

Does not recognise XRCC3 in HeLa total extracts- suggest nuclear extracts

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length Human XRCC3 (his-tagged) overexpressed in E.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 3 , CMM6

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion

Background

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Clone Reference

Masson JY et al. Genes Dev 2001; 15 (24):3296-307 Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Masson JY et al. Proc Natl Acad Sci U S A 2001; 98 (15):2388840 Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
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REVIEW

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Package List Price ($)
$ 339
Custom Conjugations