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ZC3H12A antibody [N3C3]

Anti-ZC3H12A antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX110807
Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807
Anti-ZC3H12A antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110807

Cat No. GTX110807

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, IHC
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:27322373)

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3H12A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger Ccch-Type Containing 12A,Mcpip,Mcpip-1,Mcpip1,Reg1,Dj423B22.1,Zc3H12A

Cellular Localization

Cytoplasm , Nucleus

Background

ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-ZC3H12A antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX110807

GTX110807 IHC-P Image

ZC3H12A antibody [N3C3] detects ZC3H12A protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ZC3H12A antibody [N3C3] (GTX110807) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807

GTX110807 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [N3C3] (GTX110807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZC3H12A antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110807

GTX110807 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H12A(GTX110807) antibody at 1:200 dilution.

REFERENCE
REVIEW

ZC3H12A antibody [N3C3] Cat No. GTX110807

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 23μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169