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ZNF198 antibody

Anti-ZNF198 antibody used in Western Blot (WB). GTX105550
Anti-ZNF198 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105550
Anti-ZNF198 antibody used in IHC (Paraffin sections) (IHC-P). GTX105550
Anti-ZNF198 antibody used in Western Blot (WB). GTX105550
Anti-ZNF198 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105550

Cat. No. GTX105550

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZNF198 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:500
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

Human ESC

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF198. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger MYM-type containing 2 , FIM , MYM , RAMP , SCLL , ZNF198

Background

The protein encoded by this gene is a zinc finger protein that may act as a transcription factor. The encoded protein may be part of a BHC histone deacetylase complex. Translocation of this gene with the fibroblast growth factor receptor-1 gene (FGFR1) results in a fusion gene, which may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZNF198 antibody used in Western Blot (WB). GTX105550

GTX105550 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZNF198 antibody (GTX105550) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZNF198 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105550

GTX105550 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using ZNF198(GTX105550) antibody at 1:1000 dilution.

Anti-ZNF198 antibody used in IHC (Paraffin sections) (IHC-P). GTX105550

GTX105550 IHC-P Image

ZNF198 antibody detects ZNF198 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ZNF198 antibody (GTX105550) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZNF198 antibody used in Western Blot (WB). GTX105550

GTX105550 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
5% SDS PAGE
GTX105550 diluted at 1:1000

Anti-ZNF198 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105550

GTX105550 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using ZNF198(GTX105550) antibody at 1:500 dilution.

REFERENCE

Application Reference

Yamanaka S et al. Nat Commun 2023; 14 (1) : 4683 Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation.
Yamanaka S et al. Nat Commun 2022; 13 (1) : 183 A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169