Zika virus Envelope protein antibody [GT363]

Name: Zika virus Envelope protein antibody [GT363]
Brand: GeneTex
SKU: GTX634155
Price: 399.00 USD
Rating: 5 (3 reviews)

Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155

Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155

Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155

Cat. No. GTX634155

Reference 6

Host	Mouse
Clonality	Monoclonal
Clone Name	GT363
Isotype	IgG2b
Application	WB, ICC/IF, ELISA, IHC, Sandwich ELISA
Reactivity	Zika virus
Package	100 μl ($399), 25 μl ($169)

See all Zika virus Envelope protein products

View product citations for antibody GTX634155 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:50-1:3000
ICC/IF	1:100-1:1000
ELISA	1:1000-1:10000
IHC	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX634155 / GTX133326, Detection : GTX133326 / GTX634155 / GTX133325

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Zika Virus-PRVABC59 infected C6/36 cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888405

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Envelope , ZIKV Envelope

Research Area

DATA IMAGES

GTX634155 ELISA Image

Sandwich ELISA detection of Zika virus-infected lysate using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634155 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Sample: Mock and zika virus-infected cells.
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:100.

GTX634155 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634155 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 μg/mL and GTX634155 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634155 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:500.

GTX634155 WB Image

Non-infected (–) and infected (+) Vero cell extracts (15 μg, unboiled) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) culture supernatant diluted at 1:50.

GTX634155 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634155 WB Image

Untreated (–) and treated (+) C6/36 cell extracts (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:2000.

GTX634155 WB Image

Zika virus Viral Lysate (0.5 Âμg) was separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.