GeneTex
United States (US)

Zika virus Envelope protein antibody

GTX133314_42508_20160617_WB_Zika_3_18121410_546.jpg
GTX133314_42711_20170103_IFA_18121410_421.jpg
GTX133314_42508_20160617_IFA_Zika_2_18121410_847.jpg
GTX133314_42508_20160617_IFA_Zika_18121410_360.jpg
GTX133314_42711_20170908_WB_B_Zika_18121410_600.jpg
GTX133314_42508_20160617_WB_Zika_18121410_261.jpg
GTX133314_42711_20180727_WB_Zika_18121410_261.jpg

Cat No. GTX133314

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Zika virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Zika virus Envelope-transfected 293T , Zika virus Envelope-infected C6/36
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus Envelope protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zika virus Envelope protein antibody , Zika virus Envelope protein antibody

Research Area

DATA IMAGES
GTX133314_42508_20160617_WB_Zika_3_18121410_546.jpg

GTX133314 WB Image

Mock and infected Vero and BHK-21 whole cell extracts (20 µg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133314_42711_20170103_IFA_18121410_421.jpg

GTX133314 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Envelope protein antibody (GTX133314).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX133314_42508_20160617_IFA_Zika_2_18121410_847.jpg

GTX133314 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero or BHK-21 cells using Zika virus Envelope protein antibody (GTX133314).
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133314_42508_20160617_IFA_Zika_18121410_360.jpg

GTX133314 ICC/IF Image

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus Envelope protein antibody (GTX133314).
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

GTX133314_42711_20170908_WB_B_Zika_18121410_600.jpg

GTX133314 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133314_42508_20160617_WB_Zika_18121410_261.jpg

GTX133314 WB Image

Non-infected (–) and infected (+) vero cells (15 µg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

GTX133314_42711_20180727_WB_Zika_18121410_261.jpg

GTX133314 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Zika virus Envelope protein antibody Cat No. GTX133314

Rating ( Average 4.8 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 03-Apr-2018
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-MR-766 infected BHK-21 cells
Fixation : 4% paraformaldehyde, 0.5Hr
Permeabilization /Antigen retrieval : 0.1% Triton X-100, 4°C, 2 hrHr
Primary Antibody : 1:3000, 4°C, 16Hr
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 13-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : ZIKV-infected vero cells, ZIKV-infected BHK21 cells
Blocking : 3% BSA in PBS
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, overnightHr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169