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Zika virus NS5 protein antibody

Orthogonal
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133327
Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133327
Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133327
Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133327

Cat. No. GTX133327

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

Zika-infected Vero

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Zika virus NS5 protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2800435

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 5 , Zika virus NS5 , ZIKV Non-structural protein 5 , ZIKV NS5

Research Area

DATA IMAGES

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133327

GTX133327 WB Image

Non-infected (–) and infected (+) Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133327) diluted at 1:2500.

Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133327

GTX133327 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus NS5 protein antibody (GTX133327).
Green: Zika virus NS5 protein antibody (GTX133327) diluted at 1:500.

Anti-Zika virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133327

GTX133327 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS5 protein antibody (GTX133327).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS5 protein antibody (GTX133327) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS5 protein antibody used in Western Blot (WB). GTX133327

GTX133327 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133327) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lu AY et al. J Virol 2023; 97 (5) : e0198222 Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.
Schutt WR et al. mBio 2023; 14 (5) : e0174223 ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFNβ/λ expression and promotes ZIKV persistence.
Rui Zhou et al. Bioorg Chem. 2022; 128 Repurposing of the antihistamine mebhydrolin napadisylate for treatment of Zika virus infection
Application : WB
Laura Riva et al. J Virol 2021; 95 (22) : e0099621 The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment.
Application : WB
Yang D et al. Emerg Microbes Infect 2021; 10 (1) : 1024-1037 STAT2-dependent restriction of Zika virus by human macrophages but not dendritic cells.
Application : IP
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REVIEW

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Application Reference
Lu AY et al. J Virol 2023; 97 (5):e0198222 Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.
Schutt WR et al. mBio 2023; 14 (5):e0174223 ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFNβ/λ expression and promotes ZIKV persistence.
Rui Zhou et al. Bioorg Chem. 2022; 128 Repurposing of the antihistamine mebhydrolin napadisylate for treatment of Zika virus infection
Application : WB
Laura Riva et al. J Virol 2021; 95 (22):e0099621 The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment.
Application : WB
Yang D et al. Emerg Microbes Infect 2021; 10 (1):1024-1037 STAT2-dependent restriction of Zika virus by human macrophages but not dendritic cells.
Application : IP
Sarah Goellner et al. STAR Protoc 2020; 1 (3):100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
Application : WB, ICC/IF
Cerikan B et al. Cell Rep 2020; 32 (1):107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.
Application : ICC/IF
Conde JN et al. J Virol 2020; 94 (19) NS5 Sumoylation Directs Nuclear Responses That Permit Zika Virus To Persistently Infect Human Brain Microvascular Endothelial Cells.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169