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Zika virus prM protein antibody

Anti-Zika virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133305
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

Cat. No. GTX133305

Reference 24
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus prM protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Zika virus(PRVABC59)-infected Vero , Zika virus prM-infected C6/36

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus prM protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886923

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Premembrane , Zika virus PrM , ZIKV Premembrane , ZIKV PrM

Research Area

DATA IMAGES

Anti-Zika virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133305

GTX133305 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus prM protein antibody (GTX133305).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus prM protein antibody (GTX133305) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

Non-infected (–) and infected (+) Vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika PrM antibody (GTX133305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus prM protein antibody (GTX133305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305

GTX133305 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898

REFERENCE

Application Reference

K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
He MJ et al. J Virol 2023; 97 (3) : e0180122 Key Residue in the Precursor Region of M Protein Contributes to the Neurovirulence and Neuroinvasiveness of the African Lineage of Zika Virus.
Bollman B et al. NPJ Vaccines 2023; 8 (1) : 58 An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Thompson D et al. PLoS Negl Trop Dis 2022; 16 (7) : e0010588 Zika virus-like particle vaccine fusion loop mutation increases production yield but fails to protect AG129 mice against Zika virus challenge.
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Submit a Reference

REVIEW

Zika virus prM protein antibody Cat. No. GTX133305

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus prM protein antibody used in Western Blot (WB). GTX133305
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Submit a Review
Application Reference
K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
He MJ et al. J Virol 2023; 97 (3):e0180122 Key Residue in the Precursor Region of M Protein Contributes to the Neurovirulence and Neuroinvasiveness of the African Lineage of Zika Virus.
Bollman B et al. NPJ Vaccines 2023; 8 (1):58 An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection.
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Rangel MV et al. J Virol 2022; 96 (2):e0177421 Structurally Conserved Domains between Flavivirus and Alphavirus Fusion Glycoproteins Contribute to Replication and Infectious-Virion Production.
Zhou S et al. Virol Sin 2022; 37 (5):685-694 Rottlerin plays an antiviral role at early and late steps of Zika virus infection.
Thompson D et al. PLoS Negl Trop Dis 2022; 16 (7):e0010588 Zika virus-like particle vaccine fusion loop mutation increases production yield but fails to protect AG129 mice against Zika virus challenge.
Sara B York et al. mSphere 2021;:e0019221 Zika Virus Hijacks Extracellular Vesicle Tetraspanin Pathways for Cell-to-Cell Transmission.
Application : WB
Xi Yu et al. Proc Natl Acad Sci U S A 2021; 118 (42) A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host.
Application : WB
Buchwalter RA et al. Cells 2021; 10 (12) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Buchwalter RA et al. Cells 2021; 10 (12):3335 Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.
Application : ICC/IF
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Clark AE et al. J Virol 2020; (Epub) Zika virus is transmitted in neural progenitor cells via cell-to-cell spread and infection is inhibited by the autophagy inducer trehalose.
Application : WB
Xiangling Cui et al. Front Pharmacol. 2020; 11:514313 Identification of Theaflavin-3,3'-Digallate as a Novel Zika Virus Protease Inhibitor.
Application : WB
De Lorenzo G et al. J Virol 2020; Zika virus-like particles bearing covalent dimer of envelope protein protect mice from lethal challenge.
Application : WB
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Imran M et al. Viruses 2019; 11 (11) Decanoyl-Arg-Val-Lys-Arg-Chloromethylketone: An Antiviral Compound That Acts against Flaviviruses through the Inhibition of Furin-Mediated prM Cleavage.
Application : WB
Tamhankar M et al. Virol J 2019; 16 (1):99 Directional entry and release of Zika virus from polarized epithelial cells.
Application : WB
Sabino C et al. Viruses 2019; 11 (6) Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection.
Application : WB
Arumugasaamy N et al. Ann Biomed Eng 2018; (Epub) Biomimetic Placenta-Fetus Model Demonstrating Maternal-Fetal Transmission and Fetal Neural Toxicity of Zika Virus.
Application : ICC/IF
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Estoppey D et al. Cell Rep 2017; 19 (3):451-460 The Natural Product Cavinafungin Selectively Interferes with Zika and Dengue Virus Replication by Inhibition of the Host Signal Peptidase.
Application : WB, IP
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169