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alpha 1 Antichymotrypsin antibody

Overexpression
Anti-alpha 1 Antichymotrypsin antibody used in Western Blot (WB). GTX110359
Anti-alpha 1 Antichymotrypsin antibody used in Western Blot (WB). GTX110359
Anti-alpha 1 Antichymotrypsin antibody used in Western Blot (WB). GTX110359

Cat. No. GTX110359

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all alpha 1 Antichymotrypsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Observed MW

50-70 kDa.

Positive Control

human SERPIN-transfected 293T cells , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha 1 Antichymotrypsin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885281

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family A member 3 , AACT , ACT , GIG24 , GIG25

Cellular Localization

Secreted

Background

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq]

Database

  • Gene ID: 12 SERPINA3
  • UniProt: P01011 SERPINA3

Research Area

DATA IMAGES

Anti-alpha 1 Antichymotrypsin antibody used in Western Blot (WB). GTX110359

GTX110359 WB Image

Overexpression

AACT antibody detects AACT protein by western blot analysis. Non-transfected (-) and AACT -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AACT antibody (GTX110359) diluted by 1:5000.
40 kDa may be possible degradation

Anti-alpha 1 Antichymotrypsin antibody used in Western Blot (WB). GTX110359

GTX110359 WB Image

AACT antibody detects AACT protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AACT antibody (GTX110359) diluted by 1:1000.
The observed M.W. is based on the publication: PMID: 35147994.

Anti-alpha 1 Antichymotrypsin antibody used in Western Blot (WB). GTX110359

GTX110359 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Antichymotrypsin antibody (GTX110359) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 35147994.

REFERENCE

Application Reference

Yang C.S. et al. Mol Biosyst 2017; (Epub) Proteomic Analysis of Prognostic Plasma Biomarkers in Peripheral Arterial Occlusive Disease.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169