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alpha Adaptin antibody [AC1-M11]

Anti-alpha Adaptin antibody [AC1-M11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22807
Anti-alpha Adaptin antibody [AC1-M11] used in IHC (Paraffin sections) (IHC-P). GTX22807
Anti-alpha Adaptin antibody [AC1-M11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22807
Anti-alpha Adaptin antibody [AC1-M11] used in Western Blot (WB). GTX22807

Cat. No. GTX22807

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AC1-M11

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Bovine, Chicken, Pig, Amphibians, Primate
Package
500 μl ($399)
See all alpha Adaptin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 2 μg/mL
IHC-P 1:20
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Purified bovine brain adaptor complexes.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384863

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 2 subunit alpha 1

Database

Research Area

DATA IMAGES

Anti-alpha Adaptin antibody [AC1-M11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22807

GTX22807 ICC/IF Image

ICC/IF analysis of NRK cells using GTX22807 alpha Adaptin antibody [AC1-M11].

Anti-alpha Adaptin antibody [AC1-M11] used in IHC (Paraffin sections) (IHC-P). GTX22807

GTX22807 IHC-P Image

IHC-P analysis of human heart tissue using GTX22807 alpha Adaptin antibody [AC1-M11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-alpha Adaptin antibody [AC1-M11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22807

GTX22807 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX22807 alpha Adaptin antibody [AC1-M11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-alpha Adaptin antibody [AC1-M11] used in Western Blot (WB). GTX22807

GTX22807 WB Image

WB analysis of tissue and membrane enriched extract (30 ug) of Rat Brain (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), COLO 205 (Lane 4), PC-3 (Lane 5), MCF7 (Lane 6), NIH/3T3 (Lane 7) and A-431 (Lane 8) using GTX22807 alpha Adaptin antibody [AC1-M11].
Dilution : 2 μg/ml

REFERENCE

Application Reference

Ganapathy AS et al. Autophagy 2022; 18 (9) : 2086-2103 AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.
Application : WB
Reactivity : Human
Murakami N et al. PLoS One 2012; 7 (4) : e34845 Phosphorylation by Dyrk1A of clathrin coated vesicle-associated proteins: identification of the substrate proteins and the effects of phosphorylation.
Application : IP
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399