APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:200 |
| 1-2 μg/ml |
| Assay dependent |
| 1μg per 10⁶ cells |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha adaptin found in neurons.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified adaptors.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
adaptor related protein complex 2 subunit alpha 1 , ADTAA , AP2-ALPHA , CLAPA1
Cellular Localization
Cell membrane
Background
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/templates/default/js/lightbox/images/loading.gif "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
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GTX22730 ICC/IF Image
ICC/IF analysis of U251 cells using GTX22730 alpha Adaptin antibody [AP6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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GTX22730 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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GTX22730 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX22730 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX22730 alpha Adaptin antibody [AP6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
|
REFERENCE
There are currently no references for alpha Adaptin antibody [AP6] (GTX22730). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Adaptin antibody [AP6] (GTX22730). Be the first to share your experience with this product.
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/small/GTX22730/GTX22730_397_ICC-IF_w_23060620_160.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/small/GTX22730/GTX22730_396_ICC-IF_w_23060620_664.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/small/GTX22730/GTX22730_394_ICC-IF_w_23060620_674.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/small/GTX22730/GTX22730_395_ICC-IF_w_23060620_865.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/normal/GTX22730/GTX22730_397_ICC-IF_w_23060620_160.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/normal/GTX22730/GTX22730_396_ICC-IF_w_23060620_664.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/normal/GTX22730/GTX22730_394_ICC-IF_w_23060620_674.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")
![Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730](/upload/website/prouct_img/normal/GTX22730/GTX22730_395_ICC-IF_w_23060620_865.webp "Anti-alpha Adaptin antibody [AP6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22730")