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alpha Glucosidase II antibody [C2C3], C-term

KO/KD
Anti-alpha Glucosidase II antibody [C2C3], C-term used in Western Blot (WB). GTX102205
Anti-alpha Glucosidase II antibody [C2C3], C-term used in Western Blot (WB). GTX102205
Anti-alpha Glucosidase II antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102205

Cat. No. GTX102205

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all alpha Glucosidase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

HeLa , Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950339

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucosidase II alpha subunit , G2AN , GIIA , GLUII , PKD3

Cellular Localization

Endoplasmic reticulum , Golgi apparatus , Melanosome

Background

Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.

Database

Research Area

DATA IMAGES

Anti-alpha Glucosidase II antibody [C2C3], C-term used in Western Blot (WB). GTX102205

GTX102205 WB Image

KO/KD

Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [C2C3], C-term (GTX102205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Glucosidase II antibody [C2C3], C-term used in Western Blot (WB). GTX102205

GTX102205 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102205 diluted at 1:1000

Anti-alpha Glucosidase II antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102205

GTX102205 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft , using alpha Glucosidase II(GTX102205) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169