GeneTex

  • Country / Location Selection

United States (US)

alpha Glucosidase II antibody [N1N2], N-term

KO/KD
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237
Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

Cat. No. GTX102237

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all alpha Glucosidase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , HeLa

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950338

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucosidase II alpha subunit , G2AN , GIIA , GLUII , PKD3

Cellular Localization

Endoplasmic reticulum , Golgi apparatus , Melanosome

Database

Research Area

DATA IMAGES

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

KO/KD

Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102237

GTX102237 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(GTX102237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

Sample(30 μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX102237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237

GTX102237 IHC-P Image

alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Glucosidase II antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102237

GTX102237 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using alpha Glucosidase II(GTX102237) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Glucosidase II antibody [N1N2], N-term used in Western Blot (WB). GTX102237

GTX102237 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 27283361

REFERENCE

Application Reference

Timms RT et al. Nat Commun 2016; 7 : 11786 Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for alpha Glucosidase II antibody [N1N2], N-term (GTX102237). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169