APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Raji , NIH-3T3 , JC , BCL-1 , C2C12
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.12 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Skeletal Muscle Actin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240409
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin alpha 1, skeletal muscle , ACTA , ASMA , CFTD , CFTD1 , CFTDM , MPFD , NEM1 , NEM2 , NEM3 , SHPM
Cellular Localization
Cytoplasm , cytoskeleton
Background
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX101362 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Skeletal Muscle Actin antibody (GTX101362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101362 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using alpha Actin (skeletal muscle)(GTX101362) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101362 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
10% SDS PAGE
GTX101362 diluted at 1:5000
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GTX101362 WB Image
Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
10% SDS PAGE
GTX101362 diluted at 1:1000
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GTX101362 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Skeletal Muscle Actin antibody (GTX101362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
alpha Skeletal Muscle Actin antibody
Cat. No. GTX101362
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Flow cytometry(FACS)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 26-Sep-2016
Application Tested :
FACS
Sample Species :
Hu
Sample :
HT29 cells
Blocking :
1% skim milk, 4°C, 1Hr
Fixation :
70% EtOH, -20°C, 1Hr
Permeabilization /Antigen retrieval :
70% EtOH, -20°C, 1hrHr
Primary Antibody :
1:200, 4°C, 1Hr
