GeneTex
United States (US)

alpha Tubulin antibody

Orthogonal
GTX112141_42557_20160830_IHC-P_R_18121410_405.jpg
GTX112141_42557_20160830_IHC-P_M_18121410_775.jpg
GTX112141_41094_WB_Drosophila_18121410_940.jpg
GTX112141_40905_WB_cell fraction_18121410_497.jpg
GTX112141_43236_20181005_WB_Sensitivity_19021719_835.jpg
GTX112141_42578_20181005_WB_18121410_501.jpg
GTX112141_41703_20181005_WB_M_18121410_572.jpg
GTX112141_41703_20181005_WB_R_18121410_983.jpg
GTX112141_41577_20181005_WB_18121410_563.jpg
GTX112141_43237_20180905_ICC_IF_18121410_292.jpg

Cat No. GTX112141

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Drosophila, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , HeLa cytosol fraction , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , Drosophila

Predict Reactivity

Sheep, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Database

DATA IMAGES
GTX112141_42557_20160830_IHC-P_R_18121410_405.jpg

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
alpha Tubulin antibody (GTX112141) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112141_42557_20160830_IHC-P_M_18121410_775.jpg

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
alpha Tubulin antibody (GTX112141) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112141_41094_WB_Drosophila_18121410_940.jpg

GTX112141 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.
A. 30 µg Drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin antibody (GTX112141) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_40905_WB_cell fraction_18121410_497.jpg

GTX112141 WB Image

Orthogonal

Sample (30 µg of cell lysate)
A: HeLa
B: HeLa cytosol fraction
C: HeLa nucleus fraction
10% SDS PAGE
GTX112141 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_43236_20181005_WB_Sensitivity_19021719_835.jpg

GTX112141 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_42578_20181005_WB_18121410_501.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_41703_20181005_WB_M_18121410_572.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_41703_20181005_WB_R_18121410_983.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_41577_20181005_WB_18121410_563.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_43237_20180905_ICC_IF_18121410_292.jpg

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

alpha Tubulin antibody Cat No. GTX112141

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells,Mahlavu cells,Huh6 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : Y1 cells
Blocking : 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169