GeneTex
United States (US)

alpha Tubulin antibody

GTX112141_42557_20160830_IHC-P_R_18121410_405.jpg
GTX112141_42557_20160830_IHC-P_M_18121410_775.jpg
GTX112141_41094_WB_Drosophila_18121410_940.jpg
GTX112141_40905_WB_cell fraction_18121410_497.jpg
GTX112141_42578_20181005_WB_18121410_501.jpg
GTX112141_41703_20181005_WB_M_18121410_572.jpg
GTX112141_41703_20181005_WB_R_18121410_983.jpg
GTX112141_41577_20181005_WB_18121410_563.jpg
GTX112141_43237_20180905_ICC_IF_18121410_292.jpg
GTX112141_43236_20181005_WB_Sensitivity_19021719_835.jpg

Cat No. GTX112141

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Drosophila, Chicken, Mosquito
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , HeLa cytosol fraction , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , Drosophila

Predict Reactivity

Sheep, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX112141_42557_20160830_IHC-P_R_18121410_405.jpg

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
alpha Tubulin antibody (GTX112141) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112141_42557_20160830_IHC-P_M_18121410_775.jpg

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
alpha Tubulin antibody (GTX112141) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112141_41094_WB_Drosophila_18121410_940.jpg

GTX112141 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.
A. 30 µg Drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin antibody (GTX112141) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_40905_WB_cell fraction_18121410_497.jpg

GTX112141 WB Image

Sample (30 µg of cell lysate)
A: HeLa
B: HeLa cytosol fraction
C: HeLa nucleus fraction
10% SDS PAGE
GTX112141 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_42578_20181005_WB_18121410_501.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_41703_20181005_WB_M_18121410_572.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_41703_20181005_WB_R_18121410_983.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_41577_20181005_WB_18121410_563.jpg

GTX112141 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112141_43237_20180905_ICC_IF_18121410_292.jpg

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX112141_43236_20181005_WB_Sensitivity_19021719_835.jpg

GTX112141 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

alpha Tubulin antibody Cat No. GTX112141

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells,Mahlavu cells,Huh6 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : Y1 cells
Blocking : 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169