beta-Gal antibody [N2C3]

Name: beta-Gal antibody [N2C3]
Brand: GeneTex
SKU: GTX104360
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-beta-Gal antibody [N2C3] used in Western Blot (WB). GTX104360

Anti-beta-Gal antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104360

Cat. No. GTX104360

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all beta Galactosidase products

View product citations for antibody GTX104360 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Predict Reactivity

Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta-Gal. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036345

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galactosidase beta 1 , EBP , ELNR1 , MPS4B

Cellular Localization

Isoform 1: Lysosome , Isoform 2: Cytoplasm , perinuclear region

Background

This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 2720 GLB1
UniProt: P16278 GLB1

Research Area

- Epitope Tags & Reporters

DATA IMAGES

GTX104360 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Gal antibody [N2C3] (GTX104360) diluted at 1:1000.

GTX104360 IHC-P Image

beta-Gal antibody [N2C3] detects beta-Gal protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
beta-Gal antibody [N2C3] (GTX104360) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104360 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27281159

REFERENCE

Application Reference

Chang YC et al. Mol Cancer 2017; 16 (1) : 135 Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.

Application : IHC-P

Reactivity : Human

Nagai-Okatani C et al. PLoS One 2016; 11 (6) : e0150210 Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.

Application : WB

Reactivity : Rat

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