APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , mouse brain , rat brain , HeLa
Predict Reactivity
Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.79 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888259
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4
Background
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831](/templates/default/js/lightbox/images/loading.gif "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831")
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GTX631831 ICC/IF Image
beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
The staining pattern is based on the publication:PMID: 30390091
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GTX631831 WB Image
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631831) diluted by 1:1000.
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GTX631831 WB Image
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX631831 IHC-P Image
beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX631831 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831). Be the first to share your experience with this product.
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831](/upload/website/prouct_img/small/GTX631831/GTX631831_41883_20170503_IFA_R_w_23061202_541.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831](/upload/website/prouct_img/small/GTX631831/GTX631831_41883_20141107_WB_w_23061202_393.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831](/upload/website/prouct_img/small/GTX631831/GTX631831_41883_20170309_WB_KO_watermark_w_23061202_280.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in IHC (Paraffin sections) (IHC-P). GTX631831](/upload/website/prouct_img/small/GTX631831/GTX631831_41883_20180713_IHC-P-FL_M_w_23061202_874.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in IHC (Paraffin sections) (IHC-P). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831](/upload/website/prouct_img/small/GTX631831/GTX631831_41883_20170713_WB_M_R_w_23061202_292.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831](/upload/website/prouct_img/normal/GTX631831/GTX631831_41883_20170503_IFA_R_w_23061202_541.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831](/upload/website/prouct_img/normal/GTX631831/GTX631831_41883_20141107_WB_w_23061202_393.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831](/upload/website/prouct_img/normal/GTX631831/GTX631831_41883_20170309_WB_KO_watermark_w_23061202_280.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in IHC (Paraffin sections) (IHC-P). GTX631831](/upload/website/prouct_img/normal/GTX631831/GTX631831_41883_20180713_IHC-P-FL_M_w_23061202_874.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in IHC (Paraffin sections) (IHC-P). GTX631831")
![Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831](/upload/website/prouct_img/normal/GTX631831/GTX631831_41883_20170713_WB_M_R_w_23061202_292.webp "Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831")