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beta Tubulin 3/ Tuj1 antibody [SDL.3D10]

Anti-beta Tubulin 3/ Tuj1 antibody [SDL.3D10] used in Western Blot (WB). GTX11314
Anti-beta Tubulin 3/ Tuj1 antibody [SDL.3D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11314
Anti-beta Tubulin 3/ Tuj1 antibody [SDL.3D10] used in Western Blot (WB). GTX11314

Cat No. GTX11314

Host Mouse
Clonality Monoclonal
Clone Name SDL.3D10
Isotype IgG2b
Application WB, ICC/IF, ELISA
Reactivity Human, Rat, Bovine, Pig
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:400
ICC/IF Assay dependent
ELISA Assay dependent

Note :

WB
using MDCK cells fixed in methanol

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

rat brain

Specificity/Sensitivity

The antibody specifically recognizes an epitope located on isotype III of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. The antibody can stimulate microtubule assembly when reconstituted with tubulin and either tau or MAP2.
PROPERTIES

Form

Liquid

Buffer

Ascites

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Beta 3 Class Iii,Cdcbm,Cdcbm1,Cfeom3,Cfeom3A,Feom3,Tubb4,Beta-4,Tubb3

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy-terminal. For beta-tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta- tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 3/ Tuj1 antibody [SDL.3D10] used in Western Blot (WB). GTX11314

GTX11314 WB Image

WB analysis of rat brain cytosol extract using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10].
Lane A : Antibody dilution 1:400
Lane B : Negative control (only secondary antibody)

Anti-beta Tubulin 3/ Tuj1 antibody [SDL.3D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11314

GTX11314 ICC/IF Image

ICC/IF analysis of SH-SY-5Y cells using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400(pink) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

Anti-beta Tubulin 3/ Tuj1 antibody [SDL.3D10] used in Western Blot (WB). GTX11314

GTX11314 WB Image

WB analysis of (1)U87 (2) MDBK (3) rat2 cell lysate using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400 .

REFERENCE
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REVIEW
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Package List Price ($)
$ 319