APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
| 2-4μg/ml |
|
Note :
IHC-P
Kidney shows nuclear staining in all cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89014-PEP).
Product Note
This antibody is expected to recognize isoforms 1-7 only (Numbering from NCBI RefSeq).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QRHYNDEDPEKEKR, from the internal region of the protein sequence according to NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MYB proto-oncogene, transcription factor , Cmyb , c-myb , c-myb_CDS , efg
Cellular Localization
Nucleus
Background
This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
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GTX89014 WB Image
WB analysis of MOLT4 cell lysate (35μg protein in RIPA buffer) using GTX89014 c-Myb (aa281-294) antibody, Internal.
Dilution : 2μg/ml
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GTX89014 IHC-P Image
IHC-P analysis of paraffin embedded human kidney using GTX89014 c-Myb (aa281-294) antibody, Internal.
Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 4μg/ml
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GTX89014 WB Image
WB analysis of human colon lysate using GTX89014 c-Myb (aa281-294) antibody, Internal.
Loading : 35μg protein in RIPA buffer
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GTX89014 IHC-P Image
IHC-P analysis of human kidney using GTX89014 c-Myb (aa281-294) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
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REFERENCE
There are currently no references for c-Myb (aa281-294) antibody, Internal (GTX89014). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for c-Myb (aa281-294) antibody, Internal (GTX89014). Be the first to share your experience with this product.
