c-Myc antibody [GT168]

Name: c-Myc antibody [GT168]
Brand: GeneTex
SKU: GTX628459
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-c-Myc antibody [GT168] used in Immunoprecipitation (IP). GTX628459

Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

Anti-c-Myc antibody [GT168] used in IHC (Paraffin sections) (IHC-P). GTX628459

Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459

Cat. No. GTX628459

Reference 3

Host	Mouse
Clonality	Monoclonal
Clone Name	GT168
Isotype	IgG1
Application	WB, IHC-P, FACS, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all c-Myc products

View product citations for antibody GTX628459 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human c-Myc

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888034

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Gene ID: 4609 MYC
UniProt: P01106 MYC

Research Area

DATA IMAGES

GTX628459 IP Image

Immunoprecipitation of c-Myc protein from HeLa nuclear extracts using 5 μg of c-Myc antibody [GT168] (GTX628459).
Western blot analysis was performed using c-Myc antibody [GT168] (GTX628459).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX628459 FACS Image

c-Myc antibody [GT168] (GTX628459) detects c-Myc protein by flow cytometry analysis.
Sample: NT2D1 cell fixed in 4% paraformaldehyde for 15 min on ice.
Black: Unlabelled sample was also used as a control.
Red: c-Myc antibody [GT168] (GTX628459) dilution: 1:50.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

GTX628459 IHC-P Image

c-Myc antibody [GT168] detects Myc protein at nucleus on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
c-Myc antibody [GT168] (GTX628459) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX628459 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody [GT168] (GTX628459) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628459 FACS Image

c-Myc antibody [GT168] detects C-MYC protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: c-Myc antibody [GT168] dilution: 1:50

GTX628459 WB Image

c-Myc antibody [GT168] detects Myc protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
10 % SDS-PAGE
c-Myc antibody [GT168] (GTX628459) dilution: 1:500

REFERENCE

Application Reference

Wu TH et al. Int J Mol Sci 2019; 20 (23)

Application : WB

Reactivity : Human

Yang CE et al. Bioresour Technol 2017; (Epub) Surface display of synthetic phytochelatins on Saccharomyces cerevisiae for enhanced ethanol production in heavy metal-contaminated substrates.

Yao-Yu H et al. Oncotarget 2016; (Epub) Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW