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eNOS (phospho Ser1177) antibody

Orthogonal
Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129058
Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058

Cat. No. GTX129058

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

Raji , NCI-H929

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1177 of human eNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885882

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058

GTX129058 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058

GTX129058 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058

GTX129058 WB Image

Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129058

GTX129058 ICC/IF Image

Orthogonal

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058

GTX129058 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019.PMID: 31614639

REFERENCE

Application Reference

Chang TT et al. Biomedicines 2022; 10 (8) CCL4 Deletion Accelerates Wound Healing by Improving Endothelial Cell Functions in Diabetes Mellitus.
Application : WB
Reactivity : Human, Mouse
Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.
Fan Y et al. BMC Complement Altern Med 2019; 19 (1) : 355 Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats.
Yamada T et al. Circ J 2018; 83 (1) : 232-238 Preserved Vasoconstriction and Relaxation of Saphenous Vein Grafts Obtained by a No-Touch Technique for Coronary Artery Bypass Grafting.
Application : IHC-P
Reactivity : Human
Papinska AM et al. Oxid Med Cell Longev 2018; 2018 : 1841046 Long-term administration of angiotensin (1-7) to db/db mice reduces oxidative stress damage in the kidneys and prevents renal dysfunction.
Application : IHC-P
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production