hnRNP A2B1 antibody

Name: hnRNP A2B1 antibody
Brand: GeneTex
SKU: GTX127928
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

Anti-hnRNP A2B1 antibody used in Immunoprecipitation (IP). GTX127928

Anti-hnRNP A2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127928

Cat. No. GTX127928

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all hnRNP A2B1 products

View product citations for antibody GTX127928 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616069

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein A2/B1 , HNRNPA2 , HNRNPB1 , HNRPA2 , HNRPA2B1 , HNRPB1 , IBMPFD2 , RNPA2 , SNRPB1

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]

Database

Gene ID: 3181 HNRNPA2B1
UniProt: P22626 HNRNPA2B1

Research Area

DATA IMAGES

GTX127928 WB Image

Wild-type (WT) and hnRNP A2B1 knockout (KO) HEK293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928 IP Image

hnRNPA2B1 antibody immunoprecipitates hnRNPA2B1 protein in IP experiments. IP Sample: 4500 μg A549 whole cell lysate/extract A. Immunoprecipitation of hnRNPA2B1 protein by 2 μg of hnRNPA2B1 antibody (GTX127928) B. Control with 2 μg of preimmune rabbit IgG The immunoprecipitated hnRNPA2B1 protein was detected by hnRNPA2B1 antibody diluted at 1:2000.

GTX127928 ICC/IF Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
hnRNP A2B1 antibody (GTX127928) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP A2B1 antibody (GTX127928) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127928 WB Image

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lu H.A. et al. Circ Res. 2022; 130 (1) : 112-129 Alternative Splicing Mediated by RNA-Binding Protein RBM24 Facilitates Cardiac Myofibrillogenesis in a Differentiation Stage-Specific Manner.

Application : WB

Reactivity : Human

Liu YJ et al. Neurobiol Dis 2021; 160 : 105531 A system-wide mislocalization of RNA-binding proteins in motor neurons is a new feature of ALS.

Application : WB

Reactivity : Human

Wang J et al. mBio 2020; 11 (2) RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers.

Reactivity : Human

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.

Application : WB

Reactivity : Human

Fang MY et al. Neuron 2019; (Epub) Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.

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