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hnRNP A2B1 antibody

GTX127928_42585_20190322_WB_KO_watermark_19040219_531.jpg
GTX127928_41094_IP_18121410_324.jpg
GTX127928_42613_20161101_IFA_18121410_791.jpg
GTX127928_42557_20160804_WB_M_18121410_396.jpg
GTX127928_41094_WB_R_18121410_436.jpg
GTX127928_41094_IHC_M_18121410_340.jpg
GTX127928_42557_20160830_IHC-P_M_18121410_101.jpg
GTX127928_42613_20170126_IHC-P_M_18121410_919.jpg
GTX127928_42585_20160818_WB_19021719_948.jpg

Cat No. GTX127928

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein A2/B1,Hnrnpa2,Hnrnpb1,Hnrpa2,Hnrpa2B1,Hnrpb1,Ibmpfd2,Rnpa2,Snrpb1,Hnrnpa2B1

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX127928_42585_20190322_WB_KO_watermark_19040219_531.jpg

GTX127928 WB Image

Wild-type (WT) and hnRNP A2B1 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127928_41094_IP_18121410_324.jpg

GTX127928 IP Image

hnRNPA2B1 antibody immunoprecipitates hnRNPA2B1 protein in IP experiments. IP Sample: 4500 µg A549 whole cell lysate/extract A. Immunoprecipitation of hnRNPA2B1 protein by 2 μg of hnRNPA2B1 antibody (GTX127928) B. Control with 2 μg of preimmune rabbit IgG The immunoprecipitated hnRNPA2B1 protein was detected by hnRNPA2B1 antibody diluted at 1:2000.

GTX127928_42613_20161101_IFA_18121410_791.jpg

GTX127928 ICC/IF Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

GTX127928_42557_20160804_WB_M_18121410_396.jpg

GTX127928 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000.

GTX127928_41094_WB_R_18121410_436.jpg

GTX127928 WB Image

hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
hnRNP A2B1 antibody (GTX127928) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127928_41094_IHC_M_18121410_340.jpg

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
hnRNP A2B1 antibody (GTX127928) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127928_42557_20160830_IHC-P_M_18121410_101.jpg

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP A2B1 antibody (GTX127928) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928_42613_20170126_IHC-P_M_18121410_919.jpg

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP A2B1 antibody (GTX127928) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX127928_42585_20160818_WB_19021719_948.jpg

GTX127928 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000.

REFERENCE
REVIEW

hnRNP A2B1 antibody Cat No. GTX127928

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 29-Jul-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NSC-34 cells
Blocking : 3% normal goat serum in PBS, 25°C, 1Hr
Fixation : methanol
Primary Antibody : 1:500, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169