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iNOS antibody

Orthogonal
Anti-iNOS antibody used in Western Blot (WB). GTX127952

Cat. No. GTX127952

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all iNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885665

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Database

Research Area

DATA IMAGES

Anti-iNOS antibody used in Western Blot (WB). GTX127952

GTX127952 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX127952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169