Application Note
For IHC-Fr: Use at a dilution of 1:500. For WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Product Note
Recognizes inducible nitric oxide sythase (iNOS), and does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Mouse
Immunogen
Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).
Purification
Unpurified
From polyclonal serum.
Conjugation
Unconjugated
RRID
AB_378203
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS
Cellular Localization
Cytoplasm, cytosol
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Database
Research Area