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p130Cas antibody [N2C2], Internal

KO/KD
Anti-p130Cas antibody [N2C2], Internal used in Western Blot (WB). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in Western Blot (WB). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in Western Blot (WB). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100605
Anti-p130Cas antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100605

Cat. No. GTX100605

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all p130Cas products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

H1299 , NIH-3T3 , 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p130Cas. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241140

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCAR1 scaffold protein, Cas family member , CAS , CAS1 , CASS1 , CRKAS , P130Cas

Cellular Localization

Cell junction , focal adhesion , Cytoplasm

Background

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-p130Cas antibody [N2C2], Internal used in Western Blot (WB). GTX100605

GTX100605 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p130Cas antibody [N2C2], Internal used in Western Blot (WB). GTX100605

GTX100605 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100605 diluted at 1:1000

Anti-p130Cas antibody [N2C2], Internal used in Western Blot (WB). GTX100605

GTX100605 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100605 diluted at 1:1000

Anti-p130Cas antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100605

GTX100605 ICC/IF Image

BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal (GTX100605) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-p130Cas antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100605

GTX100605 IHC-P Image

BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BCAR1 antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p130Cas antibody [N2C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100605

GTX100605 IHC-Fr Image

p130Cas antibody [N2C2], Internal detects p130Cas protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: p130Cas protein stained by p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-p130Cas antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100605

GTX100605 IHC-P Image

p130Cas antibody [N2C2], Internal detects p130Cas protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p130Cas antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100605

GTX100605 IHC-P Image

p130Cas antibody [N2C2], Internal detects p130Cas protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kumar A et al. bioRxiv 2023; Focal adhesion-derived liquid-liquid phase separations regulate mRNA translation.
Zhang X et al. J Cell Sci 2014; 127 (Pt 7) : 1394-405 N-WASP-directed actin polymerization activates Cas phosphorylation and lamellipodium spreading.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169