p130Cas (phospho Tyr165) antibody

Name: p130Cas (phospho Tyr165) antibody
Brand: GeneTex
SKU: GTX132160
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160

Anti-p130Cas (phospho Tyr165) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132160

Cat. No. GTX132160

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX132160 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HeLa , Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr165 of human p130Cas. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886583

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCAR1 scaffold protein, Cas family member , CAS , CAS1 , CASS1 , CRKAS , P130Cas

Cellular Localization

Cell junction , focal adhesion , Cytoplasm

Background

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]

Database

Gene ID: 9564 BCAR1
UniProt: P56945 BCAR1

Research Area

DATA IMAGES

GTX132160 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)

GTX132160 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132160 WB Image

GTX132160 ICC/IF Image

p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Kumar A et al. bioRxiv 2023; Focal adhesion-derived liquid-liquid phase separations regulate mRNA translation.

Yaginuma T et al. Carcinogenesis 2020; (Epub) p130Cas induces bone invasion by oral squamous cell carcinoma by regulating tumor epithelial-mesenchymal transition and cell proliferation.

Application : WB

Reactivity : Human

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