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p38 MAPK antibody [N1C3-2]

KO/KD Orthogonal
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

Cat. No. GTX110720

Reference 34
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all p38 MAPK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Sheep, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human p38 MAPK Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617720

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

KO/KD

Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110720

GTX110720 ICC/IF Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720

GTX110720 IHC-P Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720

GTX110720 IHC-P Image

p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
p38 antibody [N1C3-2] (GTX110720) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29518977

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

The data was published in the journal Mar Drugs in 2019. PMID: 31213027

REFERENCE

Application Reference

Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Zhang X et al. Chin Herb Med 2022; 14 (4) : 554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
Application : WB
Reactivity : Mouse
Fu L et al. Bioengineered 2022; 13 (5) : 12847-12862 Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells.
Application : WB
Reactivity : Human
Qian X et al. Bioengineered 2022; 13 (5) : 12583-12597 EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.
Hsu YC et al. Oxid Med Cell Longev 2022; 2022 : 1380353 Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
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Submit a Reference

REVIEW

p38 MAPK antibody [N1C3-2] Cat. No. GTX110720

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC4 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 5 μM ADC treatment for 0-120 min.
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: PANC1, Lane 2: AsPC1, Lane 3: BxPC3
Submit a Review
Application Reference
Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Zhang X et al. Chin Herb Med 2022; 14 (4):554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
Application : WB
Reactivity : Mouse
Fu L et al. Bioengineered 2022; 13 (5):12847-12862 Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells.
Application : WB
Reactivity : Human
Qian X et al. Bioengineered 2022; 13 (5):12583-12597 EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.
Hsu YC et al. Oxid Med Cell Longev 2022; 2022:1380353 Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Jihee Hong et al. J Ginseng Res 2022; 46 (3):481-488 Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.
Application : WB
Cui X et al. Dis Model Mech 2022; (Epub) Exercise suppresses mouse systemic AApoAII amyloidosis through enhancement of the p38 MAPK signaling pathway.
Application : WB
Reactivity : Mouse
Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2):1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.
Application : WB
Reactivity : Human
Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Chi-Cheng Lu et al. Exp Ther Med 2021; 22 (2):822 Dracorhodin perchlorate enhances wound healing via β-catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes.
Application : WB
Reactivity : Human
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Gwon D et al. Mol Cells 2019; 42 (12):840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : WB
Reactivity : Human
Yi-Feng Yang et al. Chinese Journal of Physiology 2019; 62 (4):139-147 Adenine decreases hypertrophic effects through interleukin-18 receptor.
Application : WB
Reactivity : Rat
Jan JS et al. Mar Drugs 2019; 17 (6) Hirsutanol A Attenuates Lipopolysaccharide-Mediated Matrix Metalloproteinase 9 Expression and Cytokines Production and Improves Endotoxemia-Induced Acute Sickness Behavior and Acute Lung Injury.
Application : WB
Reactivity : Human
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
Qi J et al. Front Mol Neurosci 2018; 11:281 Sulbactam Protects Hippocampal Neurons Against Oxygen-Glucose Deprivation by Up-Regulating Astrocytic GLT-1 via p38 MAPK Signal Pathway.
Chen CF et al. Oncol Lett 2018; 16 (5):6323-6330
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Farhat F et al. Open Access Maced J Med Sci 2018; 6 (11):1982-1985 Correlation of P38 Mitogen-Activated Protein Kinase Expression to Clinical Stage in Nasopharyngeal Carcinoma.
Application : IHC-P
Reactivity : Human
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
Application : WB
Reactivity : Human
Bonfante R et al. Neuroscience 2018; 391:120-130 The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints.
Application : WB
Reactivity : Rat
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Sarin N et al. Int J Mol Sci 2018; 19 (3) key players of * resistance: towards a systems pharmacology approach.
Application : WB
Reactivity : Human
Lin JT et al. Food Funct. 2017; 8 (3):1245-1253 Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages.
Application : WB
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Chen CC et al. PLoS One 2016; 11 (7):e0158662 The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Application : WB
Reactivity : Mouse
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Chuang YF et al. PLoS One 2015; 10 (8):e0137177 Protein Phosphatase 2A in Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Murine Lymphatic Endothelial Cells.
Application : WB
Reactivity : Mouse
Vordenbaumen S et al. BMC Immunol 2013; 14:46 Human casein alpha s1 (CSN1S1) skews in vitro differentiation of monocytes towards macrophages.
Application : WB
Reactivity : Human
Kang KH et al. Eur J Cancer 2013; 49 (12):2747-59 Enhancement role of host 12/15-lipoxygenase in melanoma progression.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169