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p53 antibody [B20.1 (BP 53.122)]

Orthogonal
Anti-p53 antibody [B20.1 (BP 53.122)] used in Western Blot (WB). GTX28590

Cat. No. GTX28590

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B20.1 (BP 53.122)

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Hamster, Monkey
Package
500 μl ($399)
See all p53 products

APPLICATION

Application Note

This antibody can be used on formalin-fixed, paraffin-embedded tissue sections at a dilution of 1:50-1:75 . Heat mediated antigen retrieval is strongly recommended for consistent and reproducible results. For western blotting use at 1:100-1:250.This antibody is not suitable for frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

Calculated MW

44 kDa. ( Note )

Positive Control

Breast Tumor - 50% of breat tumors are p53 positive, especially those lacking estrogen and/or progesterone receptors and with high proliferation index.

Product Note

This antibody reacts with wild type as well as mutant type of the p53 protein. It recognizes an epitope in the N-terminus of the human p53 protein. This antibody recognizes cell nuclei in which the p53 protein are generally localized. This antibody does not react with normal fibroblasts.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

IgG fraction

Conjugation

Unconjugated

RRID

AB_1241148

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [B20.1 (BP 53.122)] used in Western Blot (WB). GTX28590

GTX28590 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29045486

REFERENCE

Application Reference

Gusti-Ngurah-Putu EP et al. J Clin Med 2019; 8 (12) Effective Combined Photodynamic Therapy with Lipid Platinum Chloride Nanoparticles Therapies of Oral Squamous Carcinoma Tumor Inhibition.
Zera K et al. PLoS One 2017; 12 (10) : e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399