p53 antibody [C2C3], C-term

Cat. No. GTX118730

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all p53 products

Datasheet
Document

View product citations for antibody GTX118730 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HCT116 cells with 30uM cisplatin treatment for 24hr

Predict Reactivity

Rabbit, Sheep, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037588

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Database

Gene ID: 7157 TP53
UniProt: P04637 TP53

Research Area

DATA IMAGES

GTX118730 IP Image

p53 antibody immunoprecipitates p53 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of p53 protein by 2.5 μg of p53 antibody (GTX118730) 12% SDS-PAGE The immunoprecipitated p53 protein was detected by p53 antibody (GTX118730) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX118730 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 24hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
10% SDS PAGE
GTX118730 diluted at 1:1000

GTX118730 IHC-P Image

p53 antibody [C2C3], C-term detects p53 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
p53 antibody [C2C3], C-term (GTX118730) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty