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p53 antibody [DO-11]

Cat. No. GTX75258

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DO-11

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, IP

Reactivity

 Human
Package
50 μg ($399)
See all p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/1,000
IHC-P 1/1,000
IHC-Fr Assay dependent
IP Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

The antibody of clone [DO-11] recognizes mutant forms of p53 (Warnock et al. 2011) and has been used successfully for detection of p53 by western blotting (Wei et al. 2012).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human p53.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_367754

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

REFERENCE

Application Reference

Yitian Guo et al. Cell Death Dis 2021; 12 (10) : 941 p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway.
Application : WB
Reactivity : Human
Gong L et al. Oncogene 2018; (Epub) A functional interplay between Δ133p53 and ΔNp63 in promoting glycolytic metabolism to fuel cancer cell proliferation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399