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p53 antibody [Pab240]

Orthogonal
Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218
Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218
Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218
Anti-p53 antibody [Pab240] used in IHC (Paraffin sections) (IHC-P). GTX70218

Cat. No. GTX70218

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Pab240

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Mouse, Cat
Package
100 μl ($399)
See all p53 products

APPLICATION

Application Note

For IHC: Use at a dilution of 1:250 - 1:500. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. For IP: Use at 10μg/mg of lysate. By immunoprecipitation, this antibody reacts with only MUTANT p53protein under NON-DENATURING conditions. For WB: Use at a dilution of 1:2,000. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

44 kDa. ( Note )

Positive Control

HBL100 , HCT116 , 293T , A431

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Gel-purified p53 containing p53 amino acids 14-389 (derived from the pSV53C p53 cDNA clone).

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_372646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218

GTX70218 WB Image

Orthogonal

Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX70218 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218

GTX70218 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab240] (GTX70218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218

GTX70218 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab240] (GTX70218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [Pab240] used in IHC (Paraffin sections) (IHC-P). GTX70218

GTX70218 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53(GTX70218) antibody at 1:200 dilution.

REFERENCE

Application Reference

Park D et al. Korean J Physiol Pharmacol 2022; 26 (5) : 377-387 Anti-cancer effects of fenbendazole on 5-fluorouracil-resistant colorectal cancer cells.
Application : WB
Reactivity : Human
Kim JW et al. J Ethnopharmacol 2021; 280 : 114412 Anti-cancer effects of the aqueous extract of Orostachys japonica A. Berger on 5-fluorouracil-resistant colorectal cancer via MAPK signalling pathways in vitro and in vivo.
Moon D et al. Anat Cell Biol 2019; 52 (3) : 312-323 Cyclosporin A aggravates hydrogen peroxide-induced cell death in kidney proximal tubule epithelial cells.
Application : WB
Reactivity : Human
Daeun Moon et al. ACB 2019; 52 : e4 Cyclosporin A aggravates hydrogen peroxide-induced cell death in kidney proximal tubule epithelial cells.
Application : WB
Reactivity : Human
Tien YT et al. Vet J 2015; Downregulation of the KLF4 transcription factor inhibits the proliferation and migration of canine mammary tumor cells.
Application : ICC/IF
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations