APPLICATION
Application Note
IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.08% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Fusion protein, corresponding to amino acids 1-393 of Human p53.
Purification
Purified by Ammonium Sulfate precipitation.This antibody is provided as a 0.2μm sterile filtered solution.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
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GTX16121 WB Image
Each lane contains 50μg of total cellular protein separated by 10% SDS-PAGE. (1) Murine mutant p53; (2) murine wild-type p53; (3) wild-type p53 protein from human colorectal carcinoma cells (RKO); (4) elevated levels of human p53 protein following treatment of RKO cells with adriamycin (0.2 μm).
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REFERENCE
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REVIEW
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