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p62Dok (phospho Tyr362) antibody

Anti-p62Dok (phospho Tyr362) antibody used in IHC (Paraffin sections) (IHC-P). GTX50297
Anti-p62Dok (phospho Tyr362) antibody used in Western Blot (WB). GTX50297
Anti-p62Dok (phospho Tyr362) antibody used in Western Blot (WB). GTX50297

Cat. No. GTX50297

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all p62Dok products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 362 (P-I-Y(p)-D-E) derived from human p62Dok.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

docking protein 1 , P62DOK , pp62

Cellular Localization

Nucleus

Background

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-p62Dok (phospho Tyr362) antibody used in IHC (Paraffin sections) (IHC-P). GTX50297

GTX50297 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50297 p62Dok (phospho Tyr362) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-p62Dok (phospho Tyr362) antibody used in Western Blot (WB). GTX50297

GTX50297 WB Image

WB analysis of extracts from Jurkat cells untreated or treated with insulin using GTX50297 p62Dok (phospho Tyr362) antibody.

Anti-p62Dok (phospho Tyr362) antibody used in Western Blot (WB). GTX50297

GTX50297 WB Image

WB analysis of extracts from JK cells treated with G-CSF or calf intestinal phosphatase (CIP) using GTX50297 p62Dok (phospho Tyr362) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399