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p62Dok (phospho Tyr362) antibody

Orthogonal
Anti-p62Dok (phospho Tyr362) antibody used in Western Blot (WB). GTX78981
Anti-p62Dok (phospho Tyr362) antibody used in IHC (Paraffin sections) (IHC-P). GTX78981

Cat. No. GTX78981

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all p62Dok products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 362 (P-I-Yp-D-E).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11176391

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DOK1,P62DOK,docking protein 1,pp62,p62Dok,p62 DOK

Cellular Localization

Cytoplasm,Nucleus,Perinuclear region

Background

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-p62Dok (phospho Tyr362) antibody used in Western Blot (WB). GTX78981

GTX78981 WB Image

Orthogonal

WB analysis of Jurkat cell lysate using GTX78981 p62Dok (phospho Tyr362) antibody (Lane 3 and 4) and p62Dok antibody (Lane 1 and 2).

Anti-p62Dok (phospho Tyr362) antibody used in IHC (Paraffin sections) (IHC-P). GTX78981

GTX78981 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78981 p62Dok (phospho Tyr362) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399