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sRANKL antibody [12A668]

Anti-sRANKL antibody [12A668] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13582
Anti-sRANKL antibody [12A668] used in Western Blot (WB). GTX13582
Anti-sRANKL antibody [12A668] used in IHC (Paraffin sections) (IHC-P). GTX13582
Anti-sRANKL antibody [12A668] used in IHC (Paraffin sections) (IHC-P). GTX13582

Cat. No. GTX13582

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12A668

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all RANKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/ml
ICC/IF Assay dependent
IHC-P 5 μg/ml
FACS Assay dependent
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor (ligand) superfamily, member 11 , Ly109l , ODF , OPGL , RANKL , Trance

Background

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Database

Research Area

DATA IMAGES

Anti-sRANKL antibody [12A668] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13582

GTX13582 ICC/IF Image

ICC/IF analysis of mouse splenocytes using GTX13582 sRANKL antibody [12A668].
Red : primary antibody
Blue : DAPI
Dilution : 20 μg/mL

Anti-sRANKL antibody [12A668] used in Western Blot (WB). GTX13582

GTX13582 WB Image

WB analysis of human lymph node tissue lysate (in RIPA buffer) using GTX13582 sRANKL antibody [12A668].
Loading : 35μg
Dilution : 0.5 μg/ml

Anti-sRANKL antibody [12A668] used in IHC (Paraffin sections) (IHC-P). GTX13582

GTX13582 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX13582 sRANKL antibody [12A668].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

Anti-sRANKL antibody [12A668] used in IHC (Paraffin sections) (IHC-P). GTX13582

GTX13582 IHC-P Image

IHC-P analysis of human liver tissue using GTX13582 sRANKL antibody [12A668].
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409