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FRAT1 antibody [C2C3], C-term

Anti-FRAT1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110492
Anti-FRAT1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110492
Anti-FRAT1 antibody [C2C3], C-term used in Western Blot (WB). GTX110492
Anti-FRAT1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110492

Cat. No. GTX110492

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FRAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FRAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950308

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FRAT regulator of WNT signaling pathway 1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and positively regulates the Wnt signaling pathway. It may function in tumor progression and in lymphomagenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FRAT1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110492

GTX110492 IHC-P Image

FRAT1 antibody [C2C3], C-term detects FRAT1 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FRAT1 antibody [C2C3], C-term (GTX110492) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FRAT1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110492

GTX110492 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using FRAT1(GTX110492) antibody at 1:200 dilution.

Anti-FRAT1 antibody [C2C3], C-term used in Western Blot (WB). GTX110492

GTX110492 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX110492 diluted at 1:1000

Anti-FRAT1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110492

GTX110492 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using FRAT1(GTX110492) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169