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SCAMP3 antibody [N1N3]

KO/KD Orthogonal Overexpression
Anti-SCAMP3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102216
Anti-SCAMP3 antibody [N1N3] used in Western Blot (WB). GTX102216
Anti-SCAMP3 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102216
Anti-SCAMP3 antibody [N1N3] used in Western Blot (WB). GTX102216
Anti-SCAMP3 antibody [N1N3] used in Western Blot (WB). GTX102216

Cat. No. GTX102216

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SCAMP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCAMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secretory carrier membrane protein 3 , C1orf3

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene product belongs to the SCAMP family of proteins which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that the SCAMPs may function at the same site during vesicular transport rather than in separate pathways. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SCAMP3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102216

GTX102216 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adrenal gland, using SCAMP3(GTX102216) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCAMP3 antibody [N1N3] used in Western Blot (WB). GTX102216

GTX102216 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
12% SDS PAGE
GTX102216 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCAMP3 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102216

GTX102216 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SCAMP3(GTX102216) antibody at 1:1000 dilution.

Anti-SCAMP3 antibody [N1N3] used in Western Blot (WB). GTX102216

GTX102216 WB Image

Orthogonal

The data was published in the journal Cancers (Basel) in 2019. PMID: 30754729

Anti-SCAMP3 antibody [N1N3] used in Western Blot (WB). GTX102216

GTX102216 WB Image

KO/KD

The data was published in the journal Cancers (Basel) in 2019. PMID: 30754729

REFERENCE

Application Reference

Choi S et al. Sci Adv 2023; 9 (4) : eade2708 Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles.
Application : WB
Reactivity : Human
Lu JY et al. Microbiol Spectr 2021; 9 (1) : e0047521 Secretory Carrier Membrane Protein 3 Interacts with 3A Viral Protein of Enterovirus and Participates in Viral Replication.
Application : WB
Reactivity : Human
N?chel J et al. Mol Cell 2021; 81 (16) : 3275-3293.e12 An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
Tseng HW et al. Cancers (Basel) 2019; 11 (2) * treatment suppresses melanoma cell growth and motility through modulation of microrna expression.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169