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YAF2 antibody

Anti-YAF2 antibody used in Western Blot (WB). GTX115355
Anti-YAF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115355

Cat. No. GTX115355

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all YAF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human YAF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168205

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

YY1 associated factor 2

Cellular Localization

Nucleus

Background

The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-YAF2 antibody used in Western Blot (WB). GTX115355

GTX115355 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Raji
15% SDS PAGE
GTX115355 diluted at 1:500

Anti-YAF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115355

GTX115355 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using YAF2(GTX115355) antibody at 1:500 dilution.

REFERENCE

Application Reference

Su D et al. Nucleic Acids Res 2021; 49 (8) : 4421-4440 Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169