SMYD5 antibody [C2C3], C-term

Name: SMYD5 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX110518
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-SMYD5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110518

Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518

Cat. No. GTX110518

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all SMYD5 products

Datasheet
Document

View product citations for antibody GTX110518 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SMYD5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951954

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMYD family member 5 , NN8-4AG , RAI15 , RRG1 , ZMYND23

Database

Gene ID: 10322 SMYD5
UniProt: Q6GMV2 SMYD5

Research Area

DATA IMAGES

GTX110518 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SMYD5(GTX110518) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110518 IHC-P Image

SMYD5 antibody [C2C3], C-term detects SMYD5 protein at cytoplasm and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
SMYD5 antibody [C2C3], C-term (GTX110518) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110518 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD5 antibody [C2C3], C-term (GTX110518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110518 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX110518 diluted at 1:1000

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REVIEW