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B3GNT3 antibody

Anti-B3GNT3 antibody used in Western Blot (WB). GTX108928
Anti-B3GNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX108928
Anti-B3GNT3 antibody used in Western Blot (WB). GTX108928

Cat. No. GTX108928

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all B3GNT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse stomach , 293T

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human B3GNT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949713

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 , B3GAL-T8 , B3GN-T3 , B3GNT-3 , HP10328 , TMEM3 , beta3Gn-T3

Cellular Localization

Golgi apparatus membrane

Background

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-B3GNT3 antibody used in Western Blot (WB). GTX108928

GTX108928 WB Image

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
GTX108928 diluted at 1:1000

Anti-B3GNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX108928

GTX108928 IHC-P Image

B3GNT3 antibody detects B3GNT3 protein at cytosol on human colon by immunohistochemical analysis.
Sample: Paraffin-embedded colon.
B3GNT3 antibody (GTX108928) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-B3GNT3 antibody used in Western Blot (WB). GTX108928

GTX108928 WB Image

Sample (50 ug of whole cell lysate)
A: mouse stomach
10% SDS PAGE
GTX108928 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169