UBAC1 antibody [N2C3]

Cat. No. GTX120460

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all UBAC1 products

Datasheet
Document

View product citations for antibody GTX120460 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724490

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UBA domain containing 1 , GBDR1 , KPC2 , UBADC1

Cellular Localization

Cytoplasm

Background

Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

Database

Gene ID: 10422 UBAC1
UniProt: Q9BSL1 UBAC1

Research Area

DATA IMAGES

GTX120460 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using UBAC1(GTX120460) antibody at 1:200 dilution.

GTX120460 IHC-P Image

UBAC1 antibody [N2C3] detects UBAC1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
UBAC1 antibody [N2C3] (GTX120460) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX120460 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX120460 diluted at 1:1000

REFERENCE

There are currently no references for UBAC1 antibody [N2C3] (GTX120460). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for UBAC1 antibody [N2C3] (GTX120460). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty