GeneTex

  • Country / Location Selection

United States (US)

RBM14 antibody [N1N2], N-term

KO/KD Orthogonal
Anti-RBM14 antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX112293
Anti-RBM14 antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX112293
Anti-RBM14 antibody [N1N2], N-term used in Western Blot (WB). GTX112293
Anti-RBM14 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112293
Anti-RBM14 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112293

Cat. No. GTX112293

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RBM14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RBM14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721794

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding motif protein 14 , COAA , PSP2 , SIP , SYTIP1 , TMEM137

Cellular Localization

Nucleus

Background

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.

Database

Research Area

DATA IMAGES

Anti-RBM14 antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX112293

GTX112293 IHC-Wm Image

Zgc:85696 antibody detects Zgc:85696 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Zgc:85696 antibody (GTX112293) dilution: 1:50.
Anti-RBM14 antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX112293

GTX112293 IHC-Wm Image

Zgc:85696 antibody detects Zgc:85696 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Zgc:85696 antibody (GTX112293) dilution: 1:50.
Anti-RBM14 antibody [N1N2], N-term used in Western Blot (WB). GTX112293

GTX112293 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RBM14 antibody [N1N2], N-term (GTX112293) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBM14 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112293

GTX112293 ICC/IF Image

Orthogonal

The data was published in the 2018 in mSphere. PMID: 30429226

Anti-RBM14 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112293

GTX112293 ICC/IF Image

The data was published in the 2018 in mSphere. PMID: 30429226

REFERENCE

Application Reference

Narabayashi H et al. Front Mol Biosci 2022; 9 : 1005136 Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions.
Application : WB
Reactivity : Human
Beyleveld G et al. mSphere 2018; 3 (6) Nucleolar Relocalization of RBM14 by Influenza A Virus NS1 Protein.
Application : ICC/IF
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for RBM14 antibody [N1N2], N-term (GTX112293). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169