APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.92 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CAP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036425
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclase associated actin cytoskeleton regulatory protein 1 , CAP , CAP1-PEN
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CAP1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112578](/templates/default/js/lightbox/images/loading.gif "Anti-CAP1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112578")
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GTX112578 IHC-P Image
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using CAP1(GTX112578) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112578 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CAP1 antibody [C1C3] (GTX112578) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112578 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CAP1 antibody [C1C3] (GTX112578) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112578 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CAP1 antibody [C1C3] (GTX112578) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for CAP1 antibody [C1C3] (GTX112578). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CAP1 antibody [C1C3] (GTX112578). Be the first to share your experience with this product.
![Anti-CAP1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112578](/upload/website/prouct_img/small/GTX112578/GTX112578_IHC_w_23060500_825.webp "Anti-CAP1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112578")
![Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578](/upload/website/prouct_img/small/GTX112578/GTX112578_40128_20180105_WB_M_w_23060500_639.webp "Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578")
![Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578](/upload/website/prouct_img/small/GTX112578/GTX112578_40128_20180105_WB_w_23060500_421.webp "Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578")
![Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578](/upload/website/prouct_img/small/GTX112578/GTX112578_40128_20180105_WB_R_w_23060500_954.webp "Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578")
![Anti-CAP1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112578](/upload/website/prouct_img/normal/GTX112578/GTX112578_IHC_w_23060500_825.webp "Anti-CAP1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112578")
![Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578](/upload/website/prouct_img/normal/GTX112578/GTX112578_40128_20180105_WB_M_w_23060500_639.webp "Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578")
![Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578](/upload/website/prouct_img/normal/GTX112578/GTX112578_40128_20180105_WB_w_23060500_421.webp "Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578")
![Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578](/upload/website/prouct_img/normal/GTX112578/GTX112578_40128_20180105_WB_R_w_23060500_954.webp "Anti-CAP1 antibody [C1C3] used in Western Blot (WB). GTX112578")