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STK25 antibody [N1C1]

Anti-STK25 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107634
Anti-STK25 antibody [N1C1] used in Western Blot (WB). GTX107634
Anti-STK25 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107634

Cat. No. GTX107634

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all STK25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STK25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952065

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 25 , SOK1 , YSK1

Cellular Localization

Cytoplasm , Golgi apparatus

Background

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.

Database

Research Area

DATA IMAGES

Anti-STK25 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107634

GTX107634 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using STK25(GTX107634) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STK25 antibody [N1C1] used in Western Blot (WB). GTX107634

GTX107634 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK25 antibody [N1C1] (GTX107634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STK25 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107634

GTX107634 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using STK25(GTX107634) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169