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SAM68 antibody [N2C3]

Anti-SAM68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115462
Anti-SAM68 antibody [N2C3] used in Western Blot (WB). GTX115462
Anti-SAM68 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115462
Anti-SAM68 antibody [N2C3] used in Western Blot (WB). GTX115462
Anti-SAM68 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115462
Anti-SAM68 antibody [N2C3] used in Western Blot (WB). GTX115462

Cat. No. GTX115462

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SAM68 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Jurkat , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAM68. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167197

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KH RNA binding domain containing, signal transduction associated 1 , Sam68 , p62 , p68

Cellular Localization

Nucleus , Membrane

Database

Research Area

DATA IMAGES

Anti-SAM68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115462

GTX115462 ICC/IF Image

SAM68 antibody [N2C3] detects SAM68 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAM68 stained by SAM68 antibody [N2C3] (GTX115462) diluted at 1:2000.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SAM68 antibody [N2C3] used in Western Blot (WB). GTX115462

GTX115462 WB Image

SAM68 antibody [N2C3] detects SAM68 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5 % SDS-PAGE
SAM68 antibody [N2C3] (GTX115462) dilution: 1:3000

Anti-SAM68 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115462

GTX115462 IHC-P Image

SAM68 antibody [N2C3] detects SAM68 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAM68 stained by SAM68 antibody [N2C3] (GTX115462) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

Anti-SAM68 antibody [N2C3] used in Western Blot (WB). GTX115462

GTX115462 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX115462 diluted at 1:1000

Anti-SAM68 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115462

GTX115462 IHC-P Image

SAM68 antibody [N2C3] detects SAM68 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SAM68 antibody [N2C3] (GTX115462) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAM68 antibody [N2C3] used in Western Blot (WB). GTX115462

GTX115462 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SAM68 antibody [N2C3] (GTX115462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sun X et al. PLoS Biol 2016; 14 (9) : e1002543 Sam68 Is Required for DNA Damage Responses via Regulating Poly(ADP-ribosyl)ation.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169