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OVOL2 antibody

KO/KD Orthogonal Overexpression
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Anti-OVOL2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX120220
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

Cat. No. GTX120220

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all OVOL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HCT-116 , HCT-116 nuclear extract , mouse testis

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse OVOL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ovo like zinc finger 2 , 1700108N11Rik , 1810007D21Rik , M-OVO , M-OVO-A , M-OVO-B , MOVO , Ovo2 , Zfp339 , movo2

Cellular Localization

Nucleus

Background

DNA-binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. Probably acts as a transcription regulator.

Database

Research Area

DATA IMAGES

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

Orthogonal

HCT-116 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody (GTX120220) diluted at 1:1000.

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody (GTX120220) diluted at 1:500.

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

Orthogonal

The data was published in the journal Cells in 2020.PMID: 32244352

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

Overexpression

The data was published in the journal Cells in 2020.PMID: 32244352

Anti-OVOL2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX120220

GTX120220 IHC-Fr Image

KO/KD

The data was published in the journal Cells in 2020.PMID: 32244352

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

The data was published in the journal Cells in 2020.PMID: 32244352

REFERENCE

Application Reference

Jeyarajah MJ et al. Cells 2020; 9 (4) The Transcription Factor OVOL2 Represses ID2 and Drives Differentiation of Trophoblast Stem Cells and Placental Development in Mice.
Application : WB, IP, ChIP assay
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169