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WDR4 antibody

Anti-WDR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117701
Anti-WDR4 antibody used in Western Blot (WB). GTX117701
Anti-WDR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX117701

Cat. No. GTX117701

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all WDR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain 4 , TRM82 , TRMT82

Cellular Localization

Nucleus

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WDR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117701

GTX117701 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WDR4(GTX117701) antibody at 1:500 dilution.

Anti-WDR4 antibody used in Western Blot (WB). GTX117701

GTX117701 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX117701 diluted at 1:1000

Anti-WDR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX117701

GTX117701 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using WDR4(GTX117701) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169