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RPP40 antibody

Anti-RPP40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115483
Anti-RPP40 antibody used in Western Blot (WB). GTX115483

Cat. No. GTX115483

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RPP40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPP40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885372

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonuclease P/MRP subunit p40 , RNASEP1 , bA428J1.3

Cellular Localization

Nucleus , nucleolus

Background

Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.

Database

DATA IMAGES

Anti-RPP40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115483

GTX115483 ICC/IF Image

RPP40 antibody detects RPP40 protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPP40 protein stained by RPP40 antibody (GTX115483) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-RPP40 antibody used in Western Blot (WB). GTX115483

GTX115483 WB Image

RPP40 antibody detects RPP40 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RPP40 antibody (GTX115483) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169